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Topics > Biochemistry > Enzymes

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The efficiency of lactase
The goal of this experiment is to test the efficiency of lactase enzyme, with varying concentrations and temperatures of lactose.

Difficulty: High school

Measure the kinetic parameters of invertase.
Sucrose, commonly known as table sugar, is a disaccharide composed of an alpha-D-glucose molecule and a beta-D-fructose molecule linked by an alpha-1,4-glycosidic bond. When this bond is cleaved in a hydrolysis reaction, an equimolar mixture of glucose and fructose is generated. This mixture of monosaccharides is called invert sugar, which is derived from the fact that sucrose rotates plane polari

Difficulty: High school

What is the effect of Catalase on Hydrogen peroxide (H2O2)
Catalysts are molecules which speed the rate of reactions. Enzymes are special types of protein chemicals which act as catalysts for biological reactions. Enzymes can be found in all living things, plant and animal. In this activity you will investigate some of the properties of chemical reaction that is helped along by an enzyme. The enzyme used in this investigation is called catalase. Catal

Difficulty: High school

Examine the interactions of class A and C ß-lactamases as they interact with ß-lactam antibiotics.
ß-lactamases are the principal agents of bacterial resistance to many penicillins, cephalosporins, and other ß-lactam antibiotics. The main objective is to understand the interactions of class A and C ß-lactamases as they interact with ß-lactam antibiotics in order to model a non-ß-lactam inhibitor into the active site. This would stop the hydrolytic power of ß-lactamases and rejuvenate ß-lactam a

Difficulty: High school

Determine if translation and transcription of RNA in cells affects the levels of Ribonuclease H (RNase H)
Ribonuclease H is an enzyme that may be useful as a treatment for breast cancer because it can stop the production of estrogen in tumor cells. To find more about this enzyme, the purpose of this experiment was to determine if translation and transcription of RNA in cells affects the levels of Ribonuclease H (RNase H).

Difficulty: High school

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