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This science project may be completed in 1 hour if water samples are provided. Otherwise, you should allocate 1 day to complete the experiment, if water samples need to be collected. Natural water samples vary by how many living organisms can be found in a
You will need access to a pond or pond water samples. You will also need access to a compound light microscope to examine the samples.
Practice good hygiene after collecting and handling water samples from a pond. Some ponds may contain pollutants or fecal material (such as from waterfowl, fish or sewage).
Scientists group organisms into 5 or 6 kingdoms of life: animal, plant, fungi, protist and bacteria. Many of these kingdoms can be observed in a pond water sample using a microscope. These kingdoms can be distinguished by their size, shape, appendages, color and behavior. In this science project you are going to visit a local pond, collect samples and observe them under a microscope.
To gain an understanding of the kingdoms of life and the classification of aquatic organisms. To learn to use a compound light microscope to make observations of living organisms.
Close your eyes and picture a kingdom. What do you see? Do you picture a castle with walls? Are there farms in the distance? A king on his throne and knights in shining armor? What would you say if someone told you an entire kingdom can fit in a drop of water? Or that sometimes there can be as many as five kingdoms in a water droplet? Would you believe that?
The kind of kingdom that exists in a drop of water, is one that consists of living organisms that feed, breathe, move and reproduce. Biological classification is a system that scientists use to organize life into categories based on similarities and differences. Scientists group organisms into five or six different kingdoms of life. The kingdoms of life are Plant, Animal, Protist, Fungi, Eubacteria and Archaebacteria. The last two kingdoms are sometimes grouped into one kingdom called Monera.
Plants get their energy from the sun. For this reason plants tend to be the color of the light they reflect, such as green, red or orange, while the light they absorb is used to make energy. Animals get their energy by eating other living organisms. When they become hungry they eat plants, other animals, protists, fungi or even bacteria. Protists are an interesting group. They can have things in common with both plants and animals. Fungi include yeast, mold and mushrooms. Bacteria are everywhere, including on your hands and in your mouth, but you won't see them with the naked eye, as they are microscopic in size!
Salt, sugar and water are components of the food you eat every day. Their molecules are made up of basic units called atoms. Like molecules of salt, sugar and water, all living organisms are made up of basic units called cells. Cells allow the organism to breathe, eat, move and reproduce. Since the smallest cells are smaller than a pencil tip, we need to use a microscope in order to see very small organisms, which are made up of one or a few cells. Microscopes enlarge organisms that we can't see with our eyes.
In this science project you are going to identify several kingdoms of life in a water sample. First, you will collect water from a pond or lake, then bring it to your microscope for observation. In order to observe these small creatures, you will make a wet mount. Wet mounts are slides containing your sample in a solution; they are used to observe living organisms.
When observing a freshwater sample in the microscope you will encounter a few of the kingdoms. The largest organisms you may see in the microscope are in the animal kingdom. In a pond water sample, animals are larger than organisms in other kingdoms because they are multicellular. They may also have elaborate appendages, and complicated feeding behavior and movement. Most bacteria need to be stained to view in a microscope; however, in water samples long green chains of bacteria are common. These bacteria are called cyanobacteria or filamentous algae, but the latter term is also used to refer to protists or plants. Protists often have flagella (whip-like tails) or cilia (hair-like fringe). Some will have color from pigments, while others will be transparent. You may also find single-celled algae with flagella in the plant kingdom. It may be hard to distinguish these plants from protists, but protists are often more elaborate and fantastical in appearance. Protists can have amoeboid or angular shapes, more than one flagella, and noticeable eyespots.
biological classification, microscope, cells, kingdoms, wet mount
Were the organisms in Kingdom Plantae or Protista more mobile than organisms in Kingdom Animal? Why or why not?
Were the organisms in your water sample primarily from one kingdom? If so, why do you think this is? If not, why not?
Were there any kingdoms you did not observe under the microscope? Why or why not?
Questions & Answers
What differences did you notice between plants that live in the water (otherwise known as aquatic plants) and plants that live on land (terrestrial plants)?
Most aquatic plants are single-celled (unicellular), with the exception of seaweed and sea grasses, among others. This is a better adaptation for life in water because they will be less likely to sink and can move around more easily to find better living conditions, such as more light and nutrients. Terrestrial plants, like redwood trees, can get extremely large. On land being large doesn?t limit plants because they are rooted in soil, which is where they get their nutrients.
What differences did you notice between animals that live in water (aquatic animals) and animals that live on land (terrestrial animals)?
There is more than one answer to this question. Aquatic animals can also be much smaller than terrestrial animals, depending on the stage of their development. For example, copepods, which are arthropods like insects, need to be viewed with a microscope. The smaller size of aquatic animals allows them to eat all the small food that?s lower in the food chain, like protists. We also know though that aquatic animals can get very large, like the blue whale. In general, aquatic food chains are more complicated so there is more size variation in aquatic animals than there is in terrestrial animals. They can also discuss locomotion, lack of fur, lack of pigment, etc.
Make it Your Own
Do some research to answer the following questions:
Which kingdom of life has the largest living organism(s)?
Which kingdom of life has the smallest living organism(s)?
Which kingdom of life has the longest-lived organism(s)?
Which kingdom of life has the shortest-lived organism(s)?
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