Acid rain and the survival of fresh water guppies (Poecilia reticulata)
The hypothesis that the mortality rate of fresh water guppiesincreases as the acid level in the water increases, is proven to be true. Acid is toxic to the guppies and as the level of acidity of the water increases, the guppies are poisoned and die.
The problem of acid rain and pollution is very serious.
Some aquatic animals may have higher tolerance for acidic water whereas others may die and become extinct. The increase in acidity can also affect the growth and development of the species. Some of the species may not be able to hatch the eggs in acidic water. All of these will have a serious impact on the food chain and biodiversity of the ecosystem.
The experiment can also be performed using aquatic plants to substitute the fish.
You might want to consider using different species of freshwater fish for the experiment, to ascertain if different species have a different level of tolerance for acidic water.
Vinegar can be replaced by soap detergent to see if it also has a toxic effect on fish.