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Topics > Microbiology > Bacteriology

Concentration of disinfectant and survivability of bacteria

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Conclusion

The hypothesis is rejected because as the concentration of the disinfectant is decreased, the bacteria do not clearly show more resistance to the disinfectant.

It is important for us to study the impact of usage of disinfectants or antibiotics on the resistance of bacteria. Misused antibiotics can lead to unnecessary build up of resistance in the bacteria. Once bacteria become resistant, a stronger dose or different types of antibiotics have to be produced in order to fight the new strain of bacteria.

Also consider

What happens if another type of bacteria is used instead of E.Coli?

What results would you be able to observe if the incubator is set to 50 degrees Celsius?

What if another disinfectant was used?

Leave the Petri dishes in the incubator for another day. Would the zone of inhibition change? If so, how and why?

References

http://www.chemheritage.org/EducationalServices/pharm/antibiot/activity/reslab.htm
http://en.wikipedia.org/wiki/Disinfectant
http://www.scienceteacherprogram.org/biology/Webster02.html

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Complexity level:

Project cost ($):

180

Time required:

1 hour to prepare. Incubation period of 24 hours. 10 minutes for observation.

Material availability:

All of the materials mentioned should be available at the school laboratory.

Safety concerns:

Students should wear gloves and goggles at all times to prevent infection as they are dealing with microorganisms. Adult supervision is highly encouraged.