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Topics > Biochemistry > Enzymes

	Project title	Purification of soybean peroxidase
	Difficulty Level	High school
	Project page	Click here to view this science project
Description
This science fair project was performed to attempt the purification of soybean peroxidase
Instructions preview
1. For this science fair project, the independent variable is the level of catalytic activity of the commercial and purified soybean peroxidase. The dependent variable is the catalytic activity of the soybean peroxidase. This is determined by the colorimetric measurement of oxidizing pyrogallol to purpurogallin. The constants (control variables) are the pH, amount of sodium chloride and temperature of the environment, which will remain at room temperature. 2. Obtain the commercial soybean peroxidase and purify it using the BioCad Perfusion Sprint Chromatography equipment and the anion exchange polymer (10mm x 50mm). 3. Perform the ultrafiltration to remove salt by .....

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