Search for Science Fair Projects

1000 Science Fair Projects with Complete Instructions

Enzymes Science Fair Project

Purifying Soybean Peroxidase

Hard
Purifying Soybean Peroxidase | Science Fair Projects | STEM Projects
Discover how to purify soybean peroxidase, an enzyme that can help diagnose AIDS. This enzyme is more stable than horseradish peroxidase and can last up to a year without refrigeration.

Hypothesis

The hypothesis is that the soybean peroxidase can be purified by liquid chromatography.

Method & Materials

You will purify the soybean peroxidase using liquid chromatography and ultrafiltration.
You will need ethanesulfonic acid, sodium chloride, pyrogallol, hydrogen peroxide, potassium phosphate, soybean peroxidase, a BioCad Perfusion Sprint Chromatography system, an anion exchange polymer, a YM30 Amicon ultrafiltration disc, distilled water, and a Virtis Vacfreeze refrigerated condenser module.

Results

The results showed that the catalytic activity of the purified peroxidase was 60% higher compared to the unprocessed soybean peroxidase. This discovery is an important step in bringing medical assistance to the needy in poorer nations.

Why do this project?

This science project is interesting and unique because it uses soybean peroxidase, an enzyme that is more stable than horseradish peroxidase and can last up to a year without refrigeration.

Also Consider

Experiment variations to consider include testing different enzymes, such as horseradish peroxidase, and repeating the measurement at different pH solutions and different concentrations of salt.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!

Related videos

Hey there! Here are some awesome videos about this science project that we think you'll really like. They're not only super fun, but they'll also help you learn more about the science behind the project. So sit back, relax, and get ready to have some fun!!
Share this Science Project:

Related Science Fair Project Ideas

Temperature and the Catalase Enzyme
Discover how temperature affects the enzyme Catalase and how it helps convert hydrogen peroxide into oxygen and water.
Hard
Temperature and Peroxidase Enzyme
Let's explore how temperature affects the peroxidase enzyme and how it helps convert toxic hydrogen peroxide into harmless oxygen and water.
Hard
Investigating the Effects of Catalase on Hydrogen Peroxide
Let's explore how the enzyme catalase found in liver and potato affects the reaction between it and hydrogen peroxide!
Hard
Share this Science Project: