How temperature affects the Catalase enzyme
The materials required for this science fair project:
- 1 potato
- 1 blender
- 1 sheet of filter paper
- 8 beakers
- 8 test tubes
- 1 hot plate
- 1 packet of ice
- 1 bottle of hydrogen peroxide
- 1 stop watch
- 1 thermometer
- 1 pair of scissors
- 1 pair of tweezers
- 1 glass rod
- Marker pen
1. For this experiment, the independent variable is the temperature of the potato and hydrogen peroxide. The dependent variable is the reaction time in the test tube. This is determined by measuring the time it takes for the paper to float to the top of the test tube. The constants (control variables) are the amount of hydrogen peroxide used, amount of potato used and the size of the filter paper.
2. The potato is cut into small pieces and, together with some water, blended until it becomes soft.
3. The filter paper is cut into 8 pieces of small squares 5mm by 5mm each, and each piece is then dipped in the blended potato using tweezers.
4. The 8 beakers and 8 test tubes are marked 1 to 8 using the marker pen. The test tubes are also marked with a line 8mm from the bottom of the tube. The hydrogen peroxide solution is poured into the 8 test tubes up to the level of the line.
5. The 8 beakers are filled with water up to 8cm high . By adding some ice to the water, the temperature of beaker 1 is brought to 15°C and beaker 2 to 20°C. Using the hot plate, the temperatures of the water in beakers 3 to 8 are brought to 25°C, 30°C, 35°C, 40°C, 45°C and 50°C. Place the test tubes marked 1 to 8 into the beakers marked 1 to 8 accordingly for 5 minutes, to enable the hydrogen peroxide to stabilize at the same temperature.
6. Put the filter paper dipped with the blended potato into the test tubes one at a time. Push the paper into the bottom of the test tube using the glass rod. Start the stopwatch and check the time it takes for the paper to reach the surface. Record the measurements in the table below.
Be careful when using the hot plate.