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Topics > Biochemistry > Enzymes

How temperature affects the peroxidase enzyme

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Conclusion

The hypothesis that the enzyme peroxidase will become ineffective as a catalyst at high temperatures is proven to be true. The enzyme is made of protein which will become unstable at higher temperatures. At temperatures above 55°C, the protein in the enzyme peroxidase becomes thermally "denatured" and can longer combine with substrates effectively.

The peroxidase enzyme plays a very important role in reducing the toxin hydrogen peroxide which is formed in cells, into water and oxygen. Peroxidase also has industrial applications. It is used in industrial waste water treatment to remove pollutants.

Also consider

What would happen if the experiment was repeated using different amounts of hydrogen peroxide in the test tubes?

The experiment can be repeated using animal liver tissue instead of potato.

References

Enzyme - http://en.wikipedia.org/wiki/Enzyme

Peroxidase - http://en.wikipedia.org/wiki/Peroxidase

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Complexity level:

Project cost ($):

Time required:

1 hour to prepare, 1 hour for the science project experiment

Material availability:

Access to basic laboratory equipment required

Safety concerns:

Be careful when using the hot plate.