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Materials

The materials required for this science fair project:
- 15 daphnia
- 3 beakers
- a bottle of perchlorate
- a bottle of turmeric juice
- a microscope
- 3 glass slides
- 1 digital weighing scale
- 1 stopwatch
- 300ml distilled water
- 1 measuring cylinder
- 1 bottle of eye drops

Procedure

1. For this science fair project, the independent variable is the concentration of perchlorate and turmeric juice. The dependent variable is the heart rate of the daphnia. This is determined by counting the number of heart beats per minute. The constants (control variables) are the amount of perchlorate, and the amount and concentration of turmeric juice.

2. The 3 beakers are labeled as 0.1g, 1g and 10g. Three concentrations of perchlorate solution are mixed into the 3 beakers.

a. 0.1g – 100ml of distilled water is mixed with 0.1gram of perchlorate

b. 1g  100ml of distilled water is mixed with 1.0 gram of perchlorate

c. 10g – 100ml of distilled water is mixed with 10 gram of perchlorate

3. The daphnia are separated onto the 3 slides. Each slide should have 5 daphnia organisms on its surface. The slides are placed under the microscope and the heart rates of the daphnia is counted. The average number of heart beats per minute is calculated by totaling the heart rates and dividing the total by 5.

4. On the first slide, 3 drops of perchlorate from the beaker marked 0.1g  are  placed on the slide. The slide is put aside for 30 minutes giving the daphnia a chance to react to the perchlorate solution. The slide is then placed under the microscope and the average number of heart beats per minute is again recorded. The results are recorded in the table below.

5. On the same slide, 3 drops of turmeric juice  are added. The slide is again put aside for 30 minutes before counting the heart rate. The average heart rate for the 5 daphnia is calculated and recorded.

6.  Procedures 4 and 5 are repeated using the second slide for the perchlorate marked 1g and the third slide for the perchlorate marked 10g. The average heart beat is recorded in the table given below.

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Complexity level:
9
Project cost ($):
80
Time required:
1 hour to prepare, 6 hours for science fair project
Material availability:
Need
Safety concerns:

Basic safety requirements