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This experiment was performed to study the effect an energy drink has on the heartbeat rates of Daphnia. The experiment was done by exposing Daphnia to different concentrations of the Red Bull drink, mixed with water, and counting their heartbeats per minute.


Red Bull increases the heartbeat rate of Daphnia.

Scientific Terms

Taurine, caffeine, sucrose, glucose, vitamin B, glucuronolactone, phenylalanine, Daphnia, crustaceans


Red Bull

Red Bull is a very popular energy drink that is consumed all across the world. It is believed that the ingredients that are used to manufacture this drink were taken from a popular Thai energy drink called "Krating Daeng". Some of the ingredients found in this drink apparently include taurine, caffeine, sucrose, glucose, vitamin B, glucuronolactone and phenylalanine. (source:

Red Bull manufacturers claim that the energy drink can help to increase an individual’s performance, concentration, reaction time, vigilance and also improve their emotional condition.


Daphnia are tiny aquatic crustaceans also known as "water fleas". They are normally about 0.2 mm to 5.0 mm in length. They normally live in swamps, lakes, streams, rivers and ponds. Their translucent bodies make them suitable for science experiments. The Daphnia’s heartbeat can be directly observed and counted under a microscope.

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Complexity level:
Project cost ($):
Time required:
1 hour to prepare, 1 day for experiment
Material availability:
Easily found. You will need access to a lake or river to obtain the daphnia required for this experiment
Safety concerns:

Handle all laboratory equipment carefully, wearing safety gear/gloves.