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Project title Measure the kinetic parameters of invertase.
Difficulty Level High school
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Sucrose, commonly known as table sugar, is a disaccharide composed of an alpha-D-glucose molecule and a beta-D-fructose molecule linked by an alpha-1,4-glycosidic bond. When this bond is cleaved in a hydrolysis reaction, an equimolar mixture of glucose and fructose is generated. This mixture of monosaccharides is called invert sugar, which is derived from the fact that sucrose rotates plane polarized light to the right i.e., dextrorotatory, +66.5º, whereas the hydrolysis products rotates plane polarized light to the left i.e., levorotatory, -20º for the mixture (+52.5º for D(+)-glucose and -92º for D(-)-fructose). Other common disaccharides are maltose and lactose. Sucrose can be hydrolyzed in the presence of an enzyme called invertase or sucrase.  
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