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BIO 102 Lab Procedure

Plant Hormone Experiment

 

The Effect Of Different Hormones  And Hormone Concentrations On The Growth Of Radish Seeds.Your text discusses five basic plant hormones. The following web sites will also give you information on plant hormones: 

http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookPLANTHORM.html

            This site contains a basic description of the plant hormones.

http://www.biologie.uni-hamburg.de/b-online/e31/31a.htm

            This site contains a more detailed description. 

We are going to investigate the affects of two of those hormones on the growth of radish seeds. This experiment will also investigate the phenomenon of geotropism. A tropism is a growth response of a plant to an environmental influence. Phototropism is the typical growth of plants toward light that most of you have seen. This phenomenon is introduced in your textbook where the discovery of plant hormones is discussed. A response to gravity is also obvious—even with no labeling instructions, you will manage to plant seed such that the root grows and the stem grows up. This actually has nothing to do with how you place the seed in the ground. It is simply how the growing root tip and shoot tip respond to gravity. This phenomenon is called gravitropism (or sometimes geotropism). This response will also be observed in your experiment. (A third tropism is thigmotropism and is a response to touch or contact with a surface; we will not investigate thigmotropism in this experiment.)  

OBJECTIVES:

1.       Use the scientific method to investigate the effect of auxin and gibberellin on root seedling growth.

2.       Investigate  and understand geotropism in plants.

 

Plant Hormone Experiment Lab Report

 

Name______________________________________________________

Note:  Write your hypothesis for each experiment BEFORE collecting data. ALWAYS use metric measurements. 

After recording data on table below, answer the questions on this lab report sheet. (50 points total)

 

Plate

Measured Root length

Average Length

 

Seed One

Seed Two

Seed Three

Seed Four

Seed Five

Plain/No Turn

 

 

 

 

 

 

 

0.001 ppm Auxin

 

 

 

 

 

 

0.01 ppm Auxin

 

 

 

 

 

 

0.1 ppm Auxin

 

 

 

 

 

 

1.0 ppm Auxin

 

 

 

 

 

 

100 ppm Gibberellin

 

 

 

 

 

 

            Draw a graph for the average auxin data only. It may be made by computer or drawn by hand using graph paper. Any other graphs will not be accepted.  (10 pts)

Remember:

1.      Independent variable on the X-axis.

2.      Dependent variable on the Y-axis.

3.      Label both axes including units.

4.      Give the graph a title.

 

Part I    GEOTROPIC RESPONSE IN SEEDLINGS

 

  1. Write a specific hypothesis for this experiment. (2 pts)

 

 

 

 

 

2. Day and time plain agar plate was turned 90 degrees                                                                 .

 

    Days before turning                                Days after turning until measurement                        

 

Look at the turned plate and the non-turned plate.

 

3.Describe what happened to the roots after the plate was turned. Give the specific term that applies to this result. (3 pts)

 

 

 

 

4. If you can tell, describe what happened to the shoots after turning. Give the specific term that applies to this result. (2 pts)

 

 

 

 

   5. What does the non-turned plate represent in the experiment? (2 pt)

 

 

 

      6. Was your hypothesis supported?  Describe what exactly in the results supports it.  (2 pts)

 

 

 

  

B.  THE EFFECT OF HORMONES ON RADISH SEEDLINGS

 

       1. Write a hypothesis for the effect of auxins on root growth and length.  (2 pts)

 

 

 

 

       2. Write a hypothesis for the effect of gibberellin on root growth and length. (3 pts)

 

 

 

 

       3. What is the independent variable in the auxin experiment?  (1 pt)

 

 

 

       4. What is the dependent variable in the auxin experiment?  (1 pt)

 

 

 

       5. What does the non-turned plain agar plate represent in the auxin experiment?  (2 pt)

 

 

 

6. Looking at your graph for different auxin concentrations, was your hypothesis supported?  Describe    what exactly in the results supports it or refutes it. Describe any trend in the data that you see.  (4 pts)

 

 

 

 

 

 

 

 

7. Looking at the plain agar plate and the agar plate containing gibberellin, was your hypothesis supported?  Describe what exactly in the results supports it or refutes it. (2 pts)

 

 

 

 

 

 

 

       Answer the four questions that were on the lab guide sheet for this experiment.  (2 pts each)

 




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