Alligator Alley is a nickname for that section of Interstate 75 that runs from Naples on the west coast of Florida to near Fort Lauderdale on the east. Much of the highway traverses the Everglades. It was originally built as a two-lane highway connecting the two coasts of Florida, and was later connected to Interstate 75 as an east-west continuation of the highway, which otherwise ran north and south. Between 1986 and 1992 it was widened to four lanes, with many bridges designed to let water and wildlife pass underneath. This helped to reduce the environmental impact of the highway somewhat, especially upon the severely endangered Florida panther.

The name was given by the American Automobile Association during planning; they believed it would be a useless road, an "alley for alligators".

"Alligator Alley" is a grade 2/3 band piece by Michael Daugherty. It is available from Hal Leonard [1].

"Alligator Alley" is also a song by the musician Keller Williams.

See also the Britt Nichols movie Alligator Alley (2000).