Ashikaga Yoshihisa (Jp. 足利 義尚) (December 11, 1465–April 26, 1489) was the 9th shogun of the Ashikaga shogunate who reigned from 1473 to 1489 during the Muromachi period of Japan. Yoshihisa was the son of the 8th shogun Ashikaga Yoshimasa.

Since the almost 30-year-old shogun Yoshimasa had no heir by 1464, he adopted his younger brother Ashikaga Yoshimi in order to succeed him. However, Yoshihisa was born in the next year starting a struggle for succession between brothers that erupted into the Onin War starting in 1467, beginning the Sengoku period of Japanese history. In the middle of hostilities, Yoshimasa retired in 1473, relinquishing the position of Seii Taishogun to Yoshihisa.

After the Onin war, Rokkaku Takayori , the daimyo of southern Omi province, seized land and manors owned by nobles of the imperial court, temples, and shrines. In 1487, Yoshihisa led a campaign (Rokkaku Tobatsu) against Rokkaku Takayori but died of disease in 1489 leaving no heir.

Yoshihisa was followed by his cousin, the 10th shogun Ashikaga Yoshitane in the following year.