Science Fair Project Encyclopedia
Diethylpyrocarbonate
Diethylpyrocarbonate (DEPC) is used in the laboratory to remove the enzyme RNAse from water and other laboratory utensils.
Water is usually treated with 0.1% v/v diethylpyrocarbonate for at least 1 hour at 37°C and then autoclaved (at least 15 min) to inactivate traces of DEPC.
DEPC treated (and therefore free of RNAse) water is used in all handling of RNA in the laboratory, otherwise RNA would be degraded quickly by RNAse.
Last updated: 05-27-2005 11:39:23
10-26-2009 08:16:03
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The contents of this article is licensed from www.wikipedia.org under the GNU Free Documentation License. Click here to see the transparent copy and copyright details