Science Fair Project Encyclopedia
Fusion protein
A fusion protein is a protein created through genetic engineering from two or more proteins/peptides. This is achieved by removing the stop codon from the DNA sequence of the first protein, then appending the DNA sequence of the second protein in frame. That DNA sequence will then be expressed by a cell as a single protein.
This technique is often used for identification and purification of proteins, by adding a GST- or a his-tag (affinity chromatography).
10-26-2009 08:16:03
The contents of this article is licensed from www.wikipedia.org under the GNU Free Documentation License. Click here to see the transparent copy and copyright details
The contents of this article is licensed from www.wikipedia.org under the GNU Free Documentation License. Click here to see the transparent copy and copyright details