as listed in ITIS:
Subfamily Chrysopsinae :
Merycomyia
Chrysops
Neochrysops
Silvius
Subfamily Pangoniinae :
Apatolestes
Asaphomyia
Brennania
Esenbeckia
Pegasomyia
Stonemyia
Goniops
Subfamily Tabaninae :
Anacimas
Bolbodimyia
Catachlorops
Chlorotabanus
Diachlorus
Dichelacera
Holcopsis
Lepiselaga
Leucotabanus
Microtabanus
Stenotabanus
Haematopota
Agkistrocerus
Atylotus
Hamatabanus
Hybomitra
Poeciloderas
Tabanus
Whitneyomyia
Not placed:
Zophina

Among the world's largest flies are the horse-flies (family Tabanidae). Although not all the species in this family bite, these large, hairy flies are most often known as pests because of the painful bites many species can inflict on animals and humans. They occur worldwide, being absent only at extreme northern and southern latitudes.

A type of insect, horse-flies are classified in the fly order Diptera. There are approximately 3,000 species of horse-flies known worldwide, 350 of which are found in North America. At least three subfamilies are recognised:

• Chrysopsinae
• Pangoniinae
• Tabaninae
• the genus Zophina is of uncertain placement, though it has been classified among the Pangoniinae.

The two best-known types are the common horse-flies, genus Tabanus, and the deer-flies, genus Chrysops, also known as banded horse-flies because of their coloring. Both these genera give their names to subfamilies.

Adult horse-flies feed on nectar and other plant juices, but only the females also feed on blood. Males lack the necessary mouth apparatus to do so. Most horse-flies feed on mammal blood, but some species are known to feed on birds, amphibians or reptiles.

The females' primary sense for locating prey is sight, and they have large, compound eyes that serve this purpose well. The flies usually lay waiting in shady areas for prey to happen by. They are attracted to large, dark objects, and to certain animal odors and carbon dioxide. They are also attracted by motion, their eyes being well adapted to its detection.

The eyes of horse-flies are generally brightly colored, and this coloration is the primary means entomologists use to sex them.

A horse-fly's bite can be very painful. Unlike insects that pierce the skin, horse-flies have mouthparts that work like miniature knives, which they use to slash open the skin with a scissor-like motion. This causes the blood to seep out as the horse-fly licks it up. While some horse-flies are known to have venom, none is known to be dangerous to humans. When attacking humans, the flies generally prefer the head and upper body regions, going unnoticed until a bite is inflicted.

Horse-flies are most active in hot weather, mostly in summer and autumn during the daylight hours. Most species also prefer a wet climate, which makes it easier for them to breed.

Eggs are generally laid on stones close to water or on plant stems or leaves. On hatching, the larva fall into water or moist earth, feeding voraciously on invertebrates, such as snails and earthworms, and small vertebrates.

Some horse-fly species are known to transmit disease and/or parasites. A common problem in some animals, though, when large flies are abundant, is blood loss. Some animals have been known to lose up to 300 ml of blood in a single day, which can severely weaken or even kill them.