Humanist Latin is a name given to the distinctive Latin style developed by the humanist movement during the European Renaissance in the fifteenth century.

Ad fontes was the general cry of the humanists, and as such their Latin style sought to purge Latin of the medieval Latin vocabulary and stylistic accretions that it had acquired in the centuries after the fall of the Roman Empire. They looked to golden age Latin literature, and especially to Cicero in prose and Vergil in poetry, as the arbiters of Latin style. They abandoned the use of the sequence and other accentual forms of metre, and sought instead to revive the Greek formats that were used in Latin poetry during the Roman period. The humanists condemned the large body of medieval Latin literature as "gothic" — for them, a term of abuse — and believed instead that only ancient Latin from the Roman period was "real Latin".

The humanists also sought to purge written Latin of medieval developments in its orthography. They insisted, for example, that ae be written out in full wherever it occurred in classical Latin; medieval scribes often wrote e instead of ae. They were much more zealous than medieval Latin writers that t and c be distinguished; because the effects of palatalization made them homophones, medieval scribes often wrote, for example, eciam for etiam. Their reforms even affected handwriting; Humanists usually wrote Latin in a script derived from Carolingian minuscule, the ultimate ancestor of most contemporary lower-case typefaces, avoiding the black-letter scripts used in the Middle Ages. Erasmus even proposed that the then-traditional pronunciations of Latin be abolished in favour of his reconstructed version of classical Latin pronunciation.

The humanist plan to remake Latin was largely successful, at least in education. Schools now taught the humanistic spellings, and encouraged the study of the texts selected by the humanists, to the large exclusion of later Latin literature. On the other hand, while humanist Latin was an elegant literary language, it became much harder to write books about law, medicine, science or contemporary politics in Latin while observing all of the Humanists' norms about vocabulary purging and classical usage. Because humanist Latin lacked precise vocabulary to deal with modern issues, their reforms accelerated the process of turning Latin from a workday language to an object of antiquarian study. Their attempts at literary work, especially poetry, often have a strong element of pastiche. Their efforts turned Latin from a classical, but still useful language, into a truly extinct language. Latin vocabulary continued to be used by the creators of New Latin, but extensive discourses on contemporary subjects in Latin gradually ceased to be written during this period.

Important Humanist Latin authors

• Erasmus
• Petrarch
• Dante Alighieri
• Sir Thomas More
• Rudolphus Agricola
• Antonius Liber
• Poliziano
• Lorenzo Valla
• Leonardo Bruni
• Leon Battista Alberti
• Marsilio Ficino
• Pico della Mirandola
• Aeneas Sylvius
• Francesco Filelfo
• Matthaeus Fortunatus
• Guillaume Budé