Jennifer Holt (November 10, 1920 - September 21, 1997) was an American actress.

The daughter of actor Jack Holt and his wife, Margaret Woods, she was born Elizabeth Marshall Holt in Hollywood, California. She and her brother Tim would both have careers in acting.

She made her film debut using the stage name "Jacqueline Holt" in a 1941 western starring Hopalong Cassidy and went on to make forty-seven films during the 1940s. All but eight of her roles were in western films in which she appeared opposite cowboy stars such as Lash LaRue and Johnny Mack Brown.

Holt made her final film in 1949 then in 1950 co-hosted a television show called "Panhandle Pete and Jennifer" which ran for one season. During the remainder of the 1950s she made occasional guest appearances on television western series such as "The Gabby Hayes Show" and "Tales of Wells Fargo". Although the popularity of western films had faded by the mid 1950s, during the 1970s western film festivals became popular with fans of the genre and she occasionally participated as a guest star.

Jennifer Holt was married several times, living for a while in Mexico and was in Dorset in England at the time or her death in 1997.