The Prodi Commission was the European Commission from 2000 to 2004. Led by Romano Prodi, it took office on 23 January, 2000 after the Santer Commission resigned. Its planned term of office to 31 October, 2004 was extended when the initial Barroso Commission list proposal was withdrawn. It finally left office on 22 November 2004.

Besides Romano Prodi who was the President of the Commission, there were initially 19 posts which were filled as follows:

• Neil Kinnock (U.K.), Vice-President, Administrative Reform
• Loyola de Palacio (Spain), Vice-President, Relations with the Parliament, Transport & Energy
• Joaquín Almunia (Spain), Economic & Monetary Affairs
• Jacques Barrot (France), Regional Policy
• Frits Bolkestein (Netherlands), Internal Market, Taxation and Customs Union
• Philippe Busquin (Belgium), Research
• David Byrne (Ireland), Health & Consumer Protection
• Stavros Dimas (Greece), Employment and Social Affairs
• Franz Fischler (Austria), Agriculture, Rural Development & Fisheries
• Pascal Lamy (France), Trade
• Erkki Liikanen (Finland), Enterprise & Information Society
• Mario Monti (Italy), Competition
• Poul Nielson (Denmark), Development & Humanitarian Aid
• Chris Patten (U.K.), External Relations
• Viviane Reding (Luxembourg), Education and Culture
• Michaele Schreyer (Germany), Budget
• Günter Verheugen (Germany), Enlargement
• António Vitorino (Portugal), Justice and Home Affairs
• Margot Wallström (Sweden), Environment

These were augmented on 1 May, 2004 by 10 commissioners from the accession states. Each of these shared a portfolio with one of the pre-existing commissioners.

• Péter Balázs (Hungary), Regional Policy
• Joe Borg (Malta), Development & Humanitarian Aid
• Ján Figeľ (Slovakia), Enterprise & Information Society
• Dalia Grybauskaite (Lithuania), Education and Culture
• Danuta Hübner (Poland), Trade
• Siim Kallas (Estonia), Economic & Monetary Affairs
• Sandra Kalniete (Latvia), Agriculture, Rural Development & Fisheries
• Marcos Kyprianou (Cyprus), Budget
• Pavel Letička (Czech Republic), Health & Consumer Protection
• Janez Potočnik (Slovenia), Enlargement