The Gloucestershire Regiment was an infantry regiment of the British Army.

The regiment was formed in Portsmouth in 1694 by Colonel John Gibson , and was originally named the 28th (North Gloucestershire) Regiment of Foot . After Childers reforms , the regiment amalgamated with the 61st (South Gloucestershire) Regiment of Foot to form the two-battalion Gloucestershire Regiment on 1 July 1881.

The regiment saw active service in the Second Boer War from 1899-1902. The First World War began in August and during the course of the war, the regiment raised 25 battalions, seeing service on the Western Front, Gallipoli, Macedonia, the Middle East and Italy. The Second World War began After Germany's invasion of Poland on 1 September, Britain, France, and its Allies declaring war on Germany on 3 September. The 2nd battalion was involved in the Battle of France after Germany's invasion of the Low Countries on 8 May 1940, taking part in the Dunkirk evacuation. The 1st battalion saw active service in Burma against Japanese forces. The 1st battalion was later involved in the North-West Europe campaign after the invasion of Normandy on 6 June 1944.

The regiment saw service in the Korean War. After their actions at Gloster Hill during the Battle of the Imjin River in 1951, the regiment gained the nickname "The Glorious Glosters" .

The regiment was one of the British Army's most battle honoured units, and amalgamted with the Duke of Edinburgh's Royal Regiment in 1994 to form the 1st Battalion, The Royal Gloucestershire, Berkshire and Wiltshire Regiment.

In March 2005, it was announced that this regiment would merge with the Devonshire and Dorset Regiment to form the 1st Batallion, The Light Infantry.