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Topics  > Biochemistry  > Enzymes
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Demonstrate and explain what is Catalase
Enzymes are very large and complex organic molecules that are synthesized by the cell to perform very specific functions. These biological catalysts are important because they speed up the rate of the reaction they catalyze that would otherwise be too slow to support life. Catalase is an enzyme present in the cells of plants, animals and aerobic (oxygen requiring) bacteria. It promotes the convers
Difficulty: Middle school
Demonstrate the effect of the enzyme amylase on starch
In this investigation, amylase is the enzyme, and starch is the substrate, i.e. the substance it acts upon. In fact this work should add to your knowledge about the process of digestion which occurs in your body. This practical work is designed to give you some experience about: i) how enzymes work, using amylase as an example ii) what a "control" experiment tells you iii) how to carr
Difficulty: High school
Determine if translation and transcription of RNA in cells affects the levels of Ribonuclease H (RNase H)
Ribonuclease H is an enzyme that may be useful as a treatment for breast cancer because it can stop the production of estrogen in tumor cells. To find more about this enzyme, the purpose of this experiment was to determine if translation and transcription of RNA in cells affects the levels of Ribonuclease H (RNase H).
Difficulty: High school
Determining the properties of enzymes
Students quantitatively assay the activity of the enzyme peroxidase extracted from a turnip or other vegetable. They form hypotheses regarding how the rate of an enzyme-catalyzed reaction is affected by concentration of the enzyme, the pH of the reaction, temperature, inhibitors, and conduct experiments to test their hypotheses. Collected data are compiled in tables and graphs for use in a lab rep
Difficulty: High school
Examine the interactions of class A and C ß-lactamases as they interact with ß-lactam antibiotics.
ß-lactamases are the principal agents of bacterial resistance to many penicillins, cephalosporins, and other ß-lactam antibiotics. The main objective is to understand the interactions of class A and C ß-lactamases as they interact with ß-lactam antibiotics in order to model a non-ß-lactam inhibitor into the active site. This would stop the hydrolytic power of ß-lactamases and rejuvenate ß-lactam a
Difficulty: High school
Factors affecting enzyme reactions
Several other factors besides enzyme concentration can affect the rate and efficiency of enzymatic reactions, including pH, temperature, and the presence or absence of the cofactors or enzymatic inhibitors we discussed in class. In this lab, we will investigate how these factors affect enzymatic reactions by observing the effect of the two enzymes (catechol oxidase and amylase) on their respective
Difficulty: High school
Measure the kinetic parameters of invertase.
Sucrose, commonly known as table sugar, is a disaccharide composed of an alpha-D-glucose molecule and a beta-D-fructose molecule linked by an alpha-1,4-glycosidic bond. When this bond is cleaved in a hydrolysis reaction, an equimolar mixture of glucose and fructose is generated. This mixture of monosaccharides is called invert sugar, which is derived from the fact that sucrose rotates plane polari
Difficulty: High school
The efficiency of lactase
The goal of this experiment is to test the efficiency of lactase enzyme, with varying concentrations and temperatures of lactose.
Difficulty: High school
What is the dissolution rate of lactase pills
The purpose of this experiment was to determine the rate of dissolution for several dairy digestive supplements, lactase pills. I became interested in this idea because I am lactose intolerant and I wanted to buy the fastest acting pill. The information gained from this experiment would help consumers know which brand of lactase pills will dissolve fastest so they can make the best purchasin
Difficulty: Middle school
What is the effect of Catalase on Hydrogen peroxide (H2O2)
Catalysts are molecules which speed the rate of reactions. Enzymes are special types of protein chemicals which act as catalysts for biological reactions. Enzymes can be found in all living things, plant and animal. In this activity you will investigate some of the properties of chemical reaction that is helped along by an enzyme. The enzyme used in this investigation is called catalase. Catal
Difficulty: High school
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