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The hypothesis is valid because the amount of bacteria increases as the degree of opacity increases.

This study proves to be important for those countries using SODIS as a method for disinfecting drinking water. If the bottles are not well maintained, the benefits of SODIS would be lost and the people would ingest bacteria that may be harmful for them. As many diarrhea generating pathogens are found in water, the SODIS method (if carried out correctly) can help eliminate or minimize diarrhea -related deaths.

Also consider

What happens if bottles made from other material are used?

How can you make sure the PET bottles are 100% clean before carrying out the experiment?

What controls of the experiment should you take into consideration besides the ones mentioned above?


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Complexity level:
Project cost ($):
Time required:
6 hours for sunlight exposure, 24 hours incubation period
Material availability:
Tap water or rain water are acceptable sources of water. Agar plates are available at school biology laboratories.
Safety concerns:

Students must take personal safety steps by wearing gloves, mask and goggles before starting the experiment. Disinfectants should be available for standby in case of bacterial contamination. Teachers should be over watching students at all times during the experiment in case of any accidents. Students with respiratory problems should refrain from doing this experiment.