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Materials

The materials required for the science fair project:

-    Human colon colorectal adenocarcinoma tissue
-    1 tissue culture flask
-    Phosphatase assay kit
-    Well plates
-    2g of nicotine
-    400ml distilled water
-    1 spatula
-    1 digital weighing scale
-    3 beakers
-    1 measuring cylinder
-    1 pipette

Procedure

1.    For this science fair project, the independent variable is the concentration of nicotine solution. The dependent variable is the rate of proliferation of cells in the tissue sample. This is determined by performing a colorimetric analysis using the phosphatase assay kit. The constants (control variables) are the room temperature, amount tissue used and the incubation period.

2.    The Human colon colorectal adenocarcinoma tissues are colon cancer cells. They are normally obtained from the confluent tissue cultured in a flask. These are tissue that have reached a certain level of mass density and will have minimal cell multiplication activity.
3.    The tissues samples are extracted from the flask using a spatula and placed in 12 well plates. The well plates are numbered 1 to 12.

4.    The nicotine solution is prepared in the 3 beakers by adding 1mg of nicotine to 100ml water in the 1st beaker, 5mg of nicotine to 100ml water in the 2nd beaker and 10mg of nicotine to 100ml water in the 3rd beaker.

5.    Using the pipette, 3 drops of 1mg/100ml nicotine from the 1st beaker are placed in the well plates numbered 4, 5 and 6. Another 3 drops of 5mg/100 from the 2nd beaker is added to well plates 7 to 9 and 3 drops of 10mg/100 from the 3rd beaker is added to well plates 10 to 12. No nicotine is added to well plates 1 to 3.

Label Well plates Content of the beaker
None 1,2,3 None
Beaker 1 4,5,6 1mg of nicotine to 100ml water
Beaker 2 7,8,9 5mg of nicotine to 100ml water
Beaker 3 10,11,12 10mg of nicotine to 100ml water

6.    The well plates are left to incubate for 3 days. After the 3rd day, they are tested for cell proliferation or cell growth. This is done using the phosphatase test kit and colorimetric tests. The results of the tests are recorded in the table given below.

 

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Complexity level:
9
Project cost ($):
180
Time required:
1 day for preparation, 3 days for experiment
Material availability:
You will require access to a laboratory. Enlist the help of school staff to procure the necessary tissue samples
Safety concerns:

None