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How temperature affects the peroxidase enzymeFeatured science projectScience project video


This experiment was conducted to find out how different temperatures will affect the chemical reaction between the enzyme peroxidase and hydrogen peroxide.


The peroxidase enzyme will become ineffective as a catalyst at high temperatures.

Scientific Terms

Enzymes, catalase, peroxidase, hydrogen peroxide


The enzyme Peroxidase

The chemical processes that take place in cells are controlled by certain catalysts called enzymes. These enzymes are made from proteins. They help to reduce the amount of energy required to activate certain chemical processes. They do so, by binding with a substrate to produce the end products. The reduction in the required energy to activate the chemical process makes the chemical reactions happen more quickly.

The enzyme called peroxidase is one of the most common and important enzymes found in almost all living organisms. This enzyme is similar to another enzyme called catalase, but peroxidase has a slightly higher temperature tolerance. The enzymes peroxidase and catalse help to convert hydrogen peroxide into water and oxygen.

Hydrogen peroxide is a byproduct of the chemical reactions taking place in cells and is toxic to cell structures. With the help of the enzymes peroxidase and catalase, the toxic hydrogen peroxide is converted into harmless oxygen and water.

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Complexity level:
Project cost ($):
Time required:
1 hour to prepare, 1 hour for the science project experiment
Material availability:
Access to basic laboratory equipment required
Safety concerns:

Be careful when using the hot plate.