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The effect of calcium on hatching brine shrimpFeatured science projectScience project video


The materials required for this science fair project:
- 1 bottle of brine shrimp eggs
- 1 table spoon
- 4 petri dish
- 1 beaker
- 400ml distilled water
- 25mg salt
- 1 measuring cylinder
- 1 digital weighing scale
- 1 bottle of calcium
- 1 magnifying glass
- 1 black marker pen


1. For this science fair project, the independent variable is the concentration of calcium in the water – no calcium (0mg/100ml), 10mg/100ml, 20mg/100ml or 30mg/100ml. The dependent variable is the number of brine shrimp eggs that successfully hatch. This is determined by inspecting the petri dish under the magnifying glass and counting the number of hatched brine shrimp that hatch, every 12 hours. The constants (control variables) are the size of the petri dish, the salinity of the water and the room temperature.

2. The 4 petri dishes are marked A, B, C, D using  a black marker pen.

3.  400ml of distilled water and 25mg salt are mixed in the beaker. 100ml of the solution is measured using the measuring cylinder and poured into each of the 4 petri dishes.

4. 10mg of calcium is added to the petri dish marked B. 20mg and 30mg of calcium is added to the petri dishes marked C and D. The calcium powder is mixed into the solution. No calcium power is added to the petri dish labeled A.

5. A half table spoon of  brine shrimp eggs is sprinkled carefully over each of the 4 petri dishes.

6. Using the magnifying glass, the contents of the petri dishes are observed every 12 hours. The number of hatched brine shrimp  is counted and recorded.

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Complexity level:
Project cost ($):
Time required:
2 hours to prepare, 3 days for the science project experiment
Material availability:
Easily found at pet/aquarium store
Safety concerns: