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Molecular Biology Science Fair Project

Uncovering the Mystery of TAO

Hard
Uncovering the Mystery of TAO | Science Fair Projects | STEM Projects
We are on a mission to uncover the mystery of TAO. We want to know what causes the up-regulation of PGE(2) in patients with TAO and how it is related to Th1 and Th2 cytokines, growth factors, and PGE(2).

Hypothesis

The hypothesis is that TGF-B has the potential to inhibit PGE(2) and that IL-4 has a significant relationship with the up-regulation of PGE(2).

Method & Materials

You will experiment with three types of cells: Graves# orbital fibroblasts, normal orbital fibroblasts, and dermal fibroblasts (control). You will culture each type of cell in petri dishes for two weeks and then treat them with each cytokine at different time intervals.
You will need petri dishes, cytokines, lysing and scraping tools, protein assay materials, and a PGE(2) Elisa Kit Protocol.

Results

Our research experiment has led to significant insights concerning the mechanisms that lie behind TAO. Contrary to expectations, TGF-B was found to exhibit the potential to inhibit PGE(2). On the other hand, a significant relationship between the Th2 cytokine IL-4 and the up-regulation of PGE(2) was established.

Why do this project?

This science project is unique because it disproves the query that only Th1 cells are responsible for significant up-regulation of PGE2 and identifies another cytokine important for the progression of TAO.

Also Consider

Experiment variations to consider include testing different cytokines and different time intervals for treatment.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!
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