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Botany Science Fair Project

Leaf Stomata and Plant Gas Exchange

Easy
Leaf Stomata and Plant Gas Exchange | Science Fair Projects | STEM Projects
Which side of a leaf takes in gases from the air? Leaves have tiny openings called stomata (small pores that let gases move in and out). You can block these openings to find out where they are. Choose two types of plants. Coat the top of four leaves on each plant with a thick layer of Vaseline. Coat the underside of four other leaves on each plant the same way. Observe all the leaves every day for one week. Leaves coated on the underside are more likely to die. That shows the stomata are mostly on the bottom of the leaf. Without those openings, the leaf cannot take in carbon dioxide or release oxygen.

Hypothesis

The hypothesis is that the underside of a plant leaf takes in gases.

Science Concepts Learned

Stomata

Stomata are tiny openings on leaves that let gases move in and out. Most of them sit on the underside of a leaf. When you coat the bottom of a leaf with Vaseline, you block those openings — and the leaf begins to die. That shows where the stomata are and why they matter.

Method & Materials

You will coat the top and bottom of four leaves from two varieties of plants with a heavy layer of Vaseline and observe the leaves daily for one week.
You will need two varieties of plants, Vaseline, and a camera.

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Results

The results showed that three of the four leaves with Vaseline on the underside died, while only one leaf with Vaseline on the top died. This suggests that plant leaves take in gases from the bottom, not the top, of each leaf.

Why do this project?

This science project is interesting because it shows how plants exchange gases with the atmosphere and how the stomata on the underside of the leaves allow gases to move in and out.

Also Consider

Experiment variations to consider include using different types of plants or different types of coatings on the leaves.

Full project details

Additional information and source material for this project are available below.

Related video

These videos explain the science behind this project and demonstrate key concepts used in the experiment.
Stomata allow both gases and moisture to exit the leaf. This is another variation of the experiment designed to determine which side of the leaf has stomata.
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