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Water Pollution Science Fair Project

Acid Rain and Guppies

Acid Rain and Guppies | Science Fair Projects | STEM Projects
In this experiment, we'll be testing how acid rain affects guppies. We'll measure the acidity of the water in five tanks, and then add guppies to each tank. We'll then measure how many guppies are still alive after 10 hours.


The hypothesis is that the mortality rate of fresh water guppies (Poecilia reticulata) increases as the acid level in the water increases.

Method & Materials

You will need to wash and clean five fish tanks, fill each tank with one liter of water, measure the acidity of the water in each tank, add guppies to each tank, and measure how many guppies are still alive after 10 hours.
You will need 50 freshwater guppies, 5 fish tanks, 6 liters of water, 1 small bottle of vinegar, 1 syringe, 1 small fishing net, litmus paper, 1 wooden spatula, and 1 clock.


The experiment showed that the guppies in the more acidic water died faster, and the guppies in the least acidic water lived longer. This proves that acid is toxic to the guppies, and as the level of acidity of the water increases, the guppies are poisoned and die.

Why do this project?

This science project is interesting because it shows how acid rain can have a serious impact on the survival of aquatic plants and animals. It also shows how different species may have different levels of tolerance for acidic water.

Also Consider

Variations of this experiment could include using aquatic plants instead of fish, using different species of freshwater fish, or replacing vinegar with soap detergent.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!
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