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Bacteriology Science Fair Project

Bacteria and Disinfectant Resistance

Bacteria and Disinfectant Resistance | Science Fair Projects | STEM Projects
In this experiment, we'll explore how bacteria can develop resistance to disinfectants. We'll use different concentrations of disinfectant to see how bacteria react and measure the results.


The hypothesis is that the lower the concentration of disinfectant used, the higher the level of bacterial resistance.

Method & Materials

You will prepare different concentrations of disinfectant, inoculate a Petri dish with bacteria, and measure the zone of inhibition around the bacteria.
You will need a disinfectant, water, 5 Petri dishes, nutrient agar, E. coli culture, syringe, sterile cotton swab, goggles, gloves, pincers, cellophane tape, incubator, alcohol wipes, 5 50 ml beakers, 5 filter paper disks, stirrer, and marker.


The results showed that the diameter of the zone of inhibition decreased as the concentration of the disinfectant decreased. This suggests that the bacteria did not develop immunity to the disinfectant.

Why do this project?

This science project is interesting because it explores how bacteria can develop resistance to disinfectants, which is an important topic in the field of microbiology.

Also Consider

Variations to consider include using a different type of bacteria, changing the temperature of the incubator, and using a different disinfectant.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!

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