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Bacteriology Science Fair Project

Investigating Bacteria Growth Inhibition

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Investigating Bacteria Growth Inhibition | Science Fair Projects | STEM Projects
We're going to investigate how different concentrations of acid and alkaline solutions affect the growth of bacteria. We'll measure the size of the inhibition zone, or the area free of bacteria, to see how the solutions affect the bacteria.

Hypothesis

The hypothesis is that a higher concentration of acidic and alkaline solutions will result in a larger inhibition zone.

Method & Materials

You will label eight Petri dishes with different concentrations of acid and alkaline solutions, prepare different concentrations of solutions in test tubes, cut filter paper discs, and place the discs in the Petri dishes. You will then observe the Petri dishes after three days.
You will need 8 agar Petri dishes, 8 disinfected swabs, 1 bottle of disinfected water, 1 filter paper, 1 hole puncher, 9 test tubes and a rack, 1 measuring cylinder, 1 bottle of hydrochloric acid, 1 bottle of Sodium hydroxide, E. coli. bacteria culture, a pair of forceps, 1 marker pen, and a pair of gloves.

Results

The results showed that higher concentrations of acidic and alkaline solutions resulted in a larger inhibition zone. Acidic solutions were also proven to be more effective than alkaline solutions in eliminating bacteria, as shown by the larger inhibition zone in the Petri dishes that were treated with acid.

Why do this project?

This science project is interesting because it explores how different concentrations of acid and alkaline solutions affect the growth of bacteria. It also shows how acidic solutions are more effective than alkaline solutions in eliminating bacteria.

Also Consider

Consider repeating this science project using other types of acids such as sulfuric acid or alkali such as calcium carbonate. You could also repeat the experiment using other species of bacteria such as the Cocci, Spirilla or Vibro.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!

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