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Enzymes Science Fair Project

Investigating the Effects of Amylase on Starch

Investigating the Effects of Amylase on Starch | Science Fair Projects | STEM Projects
We'll investigate how amylase, an enzyme found in saliva and pancreatic juice, breaks down starch into soluble sugars. We'll use a variety of materials to observe the effects of amylase on starch.


The hypothesis is that amylase will break down starch into soluble sugars.

Method & Materials

You will label test tubes, add liquids to them, and place them in a warm waterbath. You will also test the solubility of starch, maltose, and glucose, and observe the effects of iodine and Benedict's solution on them.
You will need amylase solution, starch solution, maltose powder, glucose powder, iodine solution, Benedict's solution, 8 clean test tubes, a test-tube rack, a glass/plastic rod, a recessed tile, paper towel, a spirit marker, safety glasses, a plastic pot, and waterbaths at 37°C and 100°C.


Our experiment showed that amylase breaks down starch into soluble sugars. We observed that starch was blue-black in color when mixed with iodine, while maltose and glucose were clear orange. When Benedict's solution was added, starch produced a blue color, while maltose and glucose produced orange precipitates.

Why do this project?

This science project is interesting because it demonstrates how enzymes work to break down starch into soluble sugars. It also provides an opportunity to practice testing for starch and sugars.

Also Consider

Experiment variations to consider include testing the effects of different temperatures on the enzyme activity, and testing the effects of different concentrations of amylase on starch.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!

Related videos

Hey there! Here are some awesome videos about this science project that we think you'll really like. They're not only super fun, but they'll also help you learn more about the science behind the project. So sit back, relax, and get ready to have some fun!!
Take a look at this video on how to do the iodine test that identifies starch!
This video demonstrates the Benedict's test to look for simple sugars.
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