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Enzymes Science Fair Project

Temperature and Peroxidase Enzyme

Temperature and Peroxidase Enzyme | Science Fair Projects | STEM Projects
Temperature can have a big impact on the effectiveness of the peroxidase enzyme, which helps convert hydrogen peroxide into oxygen and water. We'll explore how temperature affects the enzyme's ability to do its job.


The hypothesis is that the peroxidase enzyme will become ineffective as a catalyst at high temperatures.

Method & Materials

You will cut a potato into small cubes, mark 8 beakers and test tubes, fill the beakers with water and adjust the temperature, add hydrogen peroxide to the test tubes, put the potato cubes into the beakers, and measure the number of bubbles formed in the test tubes.
You will need a potato, 7 beakers, 7 test tubes, a hot plate, a packet of ice, a bottle of hydrogen peroxide, a stopwatch, a thermometer, and a marker pen.


The experiment showed that bubbles were formed in test tubes 1 to 5, but no bubbles were formed in test tubes 6, 7, and 8. This proves that the hypothesis is true, and that the enzyme peroxidase becomes ineffective as a catalyst at high temperatures.

Why do this project?

This science project is interesting because it explores how temperature affects the peroxidase enzyme, which is an important enzyme found in almost all living organisms. It helps to convert hydrogen peroxide into water and oxygen, which is essential for cell health.

Also Consider

Variations of this experiment could include using different amounts of hydrogen peroxide in the test tubes, or using animal liver tissue instead of potato.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!

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