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Enzymes Science Fair Project

Purifying Soybean Peroxidase

Purifying Soybean Peroxidase | Science Fair Projects | STEM Projects
Discover how to purify soybean peroxidase, an enzyme that can help diagnose AIDS. This enzyme is more stable than horseradish peroxidase and can last up to a year without refrigeration.


The hypothesis is that the soybean peroxidase can be purified by liquid chromatography.

Method & Materials

You will purify the soybean peroxidase using liquid chromatography and ultrafiltration.
You will need ethanesulfonic acid, sodium chloride, pyrogallol, hydrogen peroxide, potassium phosphate, soybean peroxidase, a BioCad Perfusion Sprint Chromatography system, an anion exchange polymer, a YM30 Amicon ultrafiltration disc, distilled water, and a Virtis Vacfreeze refrigerated condenser module.


The results showed that the catalytic activity of the purified peroxidase was 60% higher compared to the unprocessed soybean peroxidase. This discovery is an important step in bringing medical assistance to the needy in poorer nations.

Why do this project?

This science project is interesting and unique because it uses soybean peroxidase, an enzyme that is more stable than horseradish peroxidase and can last up to a year without refrigeration.

Also Consider

Experiment variations to consider include testing different enzymes, such as horseradish peroxidase, and repeating the measurement at different pH solutions and different concentrations of salt.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!

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