Does warming a lactose solution help the enzyme lactase break it down faster? Lactase splits lactose (milk sugar) into simpler sugars like glucose. Temperature and concentration both change how well it works. You prepare lactose solutions at different concentrations. Then you heat each one in small steps of five degrees. At every step, you add lactase enzyme and test for glucose with test strips. The results show a clear pattern. At lower lactose concentrations, the enzyme reaches peak glucose output at a lower temperature. Heat weakens the bonds in lactose, so the enzyme has less work to do.