
Catalase Reaction Rate in Liver and Potato
Medium
How fast does the enzyme catalase break down hydrogen peroxide? Catalase is found in living tissues like liver and potato. When it meets hydrogen peroxide, it triggers a rapid fizzing reaction that produces oxygen gas.
You place a small amount of liver or potato extract in a test tube. Then you pour in diluted hydrogen peroxide and immediately start timing. The reaction creates a rising column of froth. You measure its height with a ruler.
The reaction rate equals froth height divided by time. You can test different variables like temperature to see how they change the speed. Keeping extract on ice before use helps preserve the enzyme.
Hypothesis
The hypothesis is that the rate of reaction between catalase and hydrogen peroxide will be affected by different variables.
Method & Materials
You will measure out 0.25 ml of extract, 1 ml of 3% hydrogen peroxide and 1 ml of distilled water. Then, you will pour the peroxide into the liver extract tube and immediately begin timing and measuring the distance from the bottom of the tube to the line of maximum height.
You will need liver extract or pieces, potato extract or pieces, 3% Hydrogen Peroxide, test tubes, test tube racks, stopwatch, millimeter ruler, beakers or cups, and a disposable pipette.
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See what’s includedResults
Our experiment has revealed that the rate of reaction between catalase and hydrogen peroxide is affected by different variables. An interesting observation is that the rate of reaction increases with temperature.
Why do this project?
This science project is interesting and unique because it allows us to explore the effects of catalase on hydrogen peroxide in a controlled environment.
Also Consider
Variations to consider include testing the reaction in different temperatures (0, 40, 60, 80) and testing different concentrations of hydrogen peroxide.
Full project details
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