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Bacteriology Science Fair Project

Dog Saliva and Bacterial Growth Inhibition

Hard
Dog Saliva and Bacterial Growth Inhibition | Science Fair Projects | STEM Projects
You have probably heard that a dog licking a wound helps it heal. Can dog saliva really stop bacteria from growing? This project puts that idea to the test with two experiments. In the first experiment you swab bacteria from surfaces like hands and tables onto agar dishes. After the bacteria grow you make a broth from each type. You place the broth and dog saliva together in new dishes and watch for two days. In the second experiment you grow a lawn of E. coli on agar plates. You collect saliva from 102 dogs by touching sterile filter paper to their upper gums. You place each sample on the E. coli lawn and look for rings of inhibition (zones where bacteria stopped growing). About 16 percent of samples showed clear inhibition.

Hypothesis

The hypothesis is that dog saliva can kill bacteria.

Method & Materials

You will collect samples of bacteria from around your school, and then place them in petri dishes with dog saliva.
You will need sterile cotton swabs, petri dishes, agar, and dog saliva.

Results

The results of this experiment suggest that dog saliva does have some antibacterial properties, although it is not a surefire way to kill bacteria. In one experiment, 16% of the samples showed inhibition of bacteria growth.

Why do this project?

This science project is interesting because it explores the potential of dog saliva as a natural antibiotic.

Also Consider

Variations to consider include testing different types of bacteria, or testing the saliva of different breeds of dogs.

Full project details

Additional information and source material for this project are available below.

Related video

These videos explain the science behind this project and demonstrate key concepts used in the experiment.
In this video, you'll learn how to grow bacteria on special plates called agar plates. But don't worry, you don't have to make the plates yourself - you can buy them already made! Then, at the 1-minute mark, you'll see how to collect bacteria samples. After that, get ready to test if dog saliva can stop bacteria from growing!
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