Bacteriology is the study of microscopic living things called bacteria. Scientists are learning more about how to promote the growth of good bacteria and fight bad ones to improve our health. Additionally, bacteria are used in various industries to produce useful compounds.

While antibiotics are very powerful in the cure of many bacterial diseases, some bacteria have developed ways to resist antibiotics. The extensive use of antibiotics (unfortunately including the over-prescription and misuse of these medications) has resulted in the evolution of bacteria that are not be killed by antibiotics and these bacteria once again threaten health and life. Diseases that were virtually eliminated with the introduction of antibiotics are becoming more and more difficult to cure. Each and every one of us has the ability to help with this ever increasing problem.

Can bacteria survive an antibiotic that once killed them? Some bacteria develop resistance after repeated exposure. This two-part project tests how antibiotics affect bacterial growth on agar plates.

First you press everyday objects onto agar plates. One plate contains an antibiotic in the medium. You compare growth after a week to see which bacteria survive. In the second experiment you streak E. coli across a plate and place disks of penicillin G and tetracycline on it. After incubation you measure the clear zone around each disk.

The size of each zone shows whether E. coli is sensitive or resistant to that antibiotic.

Purpose: Does bacteria become more resistant to an antibiotic when exposed repeatedly to it?

BACKGROUND:

Antibiotics are substances that are produced by molds or bacteria and that kill
or inhibit the growth of other microorganisms.   Antibiotics specifically attack bacteria without harming cells belonging to the
organism that produced them. Antibiotics such as penicillin kill bacteria by inhibiting them from making cell walls that are needed for their survival.
Without their cell wall the contents of the cells leak out and the cell is
destroyed. Human and animal cells don’t require a cell wall in order to survive,
thus these antibiotics do not damage them. While antibiotics are very powerful in the cure of many bacterial diseases, some
bacteria have developed ways to resist antibiotics. The extensive use of
antibiotics (unfortunately including the over-prescription and misuse of these
medications) has resulted in the evolution of bacteria that are not be killed by
antibiotics and these bacteria once again threaten health and life. Diseases
that were virtually eliminated with the introduction of antibiotics are becoming
more and more difficult to cure. Each and every one of us has the ability to
help with this ever increasing problem.


EXPERIMENT# 1 MATERIALS:

• One sterile agar plate labeled with letter A (for antibiotic added)
• One sterile agar plate without writing on it (antibiotic free plate)
• Objects of your choice to test for the presence of microorganisms: a penny,
keys, pens, etc.
• Q-tips (to get bacteria from teeth, sandwiches, fruit juice, etc.)
• toothpicks
• markers for labeling
 
EXPERIMENT# 1 PROCEDURE (FIRST WEEK)

1: Get one of each type of agar plate and label it on the bottom with your name,
date, and the origin of your bacteria. You may want to divide the plate into
sections by drawing lines on the bottom of the plate (on the outside) and trying
different objects in each section.

2: Open the cover of the plate and gently press your object on the surface of
the agar. Be careful not to press too hard – you don’t want to press through the
agar. Make sure to label what object you used in each section. If you want to
try testing food, drinks, your teeth, etc., then wet the Q-tip with the object,
and rub the Q-tip on the surface of the agar. You can also use a toothpick.
 
3. Cover the plate as soon as you remove your object, the Q-top, or the
toothpick. You want to have the lid open for as little time as possible so
microorganisms from the air do not fall on the plate.

4: Write a hypothesis about what you think will happen on each of your plates.

Your plate will be left at room temperature until next week so that any bacteria
that are present can grow into colonies.
 
EXPERIMENT# 1 PROCEDURE (SECOND WEEK)

1. Record what you observed on each of your plates.

2. Give an explanation for why you did or did not see growth on the plate that
had the antibiotic added to the medium.

3. Discuss how you might be able to help preventing a further increase in
antibiotic resistant microorganisms.

EXPERIMENT# 2

Because of the increased occurrence of antibiotic resistant bacteria a doctor

might test which antibiotics the disease causing bacterium is sensitive to
before writing a prescription. Today we will use an assay, similar to the one
the doctor would perform, to determine whether the Escherichia colibacterium (a
harmless bacterium growing in our gut) is sensitive or resistant to the
antibiotics penicillinG and tetracycline.

EXPERIMENT# 2 MATERIALS:

- One sterile agar plate
- A culture of Escherichia coli bacteria
- A sterile swab
- One tetracycline antibiotic disc
- One penicillinG antibiotic disc
- Forceps 

EXPERIMENT# 2 PROCEDURE (FIRST WEEK)

1: Get one agar plate and label it on the bottom with your name and the date.

Take a swab and soak it into the liquid bacterial culture. Open the cover of the
plate and gently streak the swab numerous times across the agar so that bacteria
will grow on the whole plate. Use forceps to remove an antibiotic disc and place
it on one side of the plate (approximately 1/2 inch from the side). On the
opposite side place the second antibiotic disc.

2: Cover the plate as soon as you placed your antibiotic discs on them. Your
plate will be incubated at 37C so that the Escherichia coli bacteria can grow on
the plate.

EXPERIMENT# 2 PROCEDURE (SECOND WEEK)

1: Record what you observed on the plate

2: Give an explanation for why you did or did not see growth on around the
discs.

 

Antibiotic Resistance in E. coli

Does bacteria become more resistant to an antibiotic when exposed repeatedly to it?

<h1><strong>1000 Science Fair Project Ideas by Subject</strong></h1><p>Looking for a science fair project idea? Each of our science fair project idea has complete instructions with video. Get ideas for your science projects, find extra resources for your class' scientific projects, or try a fun experiment at home! It doesn't matter what grade you're in, there's something for everyone!</p><p>You can find your perfect science fair project from the different science subjects below or from our <a href="https://www.all-science-fair-projects.com/category0.html#bottom">science fair project idea lists!</a></p>

<h2>Science Fair Project Idea Lists</h2><p>Looking for other ways to find good ideas for science fair projects? Check out these lists of science projects!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>Easy Science Fair Projects</h3><p><img src="/images2/flex/176_popular 2 (2).png" data-size="100" data-float="left" style="width:100px; float:left">Explore the exciting world of science with an easy science fair project that you can do at home using simple materials. Any one of these easy project ideas will teach you important scientific concepts and help you have fun while learning. An easy science fair project is a great way to introduce the wonders of science to kids and adults alike!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>Changing the Color of Flowers</p></li><li><p>Baking Soda Volcano</p></li><li><p>Swimming Raisins</p></li><li><p>Egg in Vinegar</p></li><li><p>Color of Skittles Experiment</p></li><li><p>Jumping Rice Krispies</p></li><li><p>Mixing Colors of Light</p></li><li><p>Camouflage and Hide</p></li><li><p>Light and Dark on Seed Germination</p></li><li><p>Make a Parachute</p></li></ul><p>More science project details at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/12">Easy Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>The Top 10 Best Science Fair Projects</h3><p><img src="/images2/flex/177_popular 2 (3).png" data-size="100" data-float="left" style="width:100px; float:left">To find the best science fair project for you, we've picked the best science projects from 10 different science categories, all with very interesting topics. If you're looking for more science projects at another difficulty level or topic, you can explore science fair project ideas by grade level at the top of this page!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>Jet Lag in Hamsters (circadian rhythm, Biology science fair project)</p></li><li><p>Egg with Vinegar (acid-base reaction, Chemistry science fair project)</p></li><li><p>Galileo's Inclined Plane Experiment (acceleration, Physics science fair project)</p></li><li><p>Plant's Favorite Color for Photosynthesis (Photosynthesis, Botany science fair project)</p></li><li><p>Color and Taste (color perception, Psychology science fair project)</p></li><li><p>Does Dog Saliva Kill Bacteria? (antibacterial, Microbiology science fair project)</p></li><li><p>Decomposing Plastic Spoons (biodegradable materials, Environmental Science science fair project)</p></li><li><p>Super Hero Egg Shells (structural engineering, Engineering science fair project)</p></li><li><p>Music Math: Playing Notes with Equations (music, Math science fair project)</p></li><li><p>Temperature's Effect on Seawater (climate change, Earth Science science fair project)</p></li></ul><p>More science project details at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/13">Best Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>Cool Science Fair Projects</h3><p><img src="/images2/flex/178_popular 2.png" data-size="100" data-float="left" style="width:100px; float:left">Get ready to be amazed by super cool science projects that you can do right at home! With just a few simple things, you can be fascinated by science and have tons of fun with these popular science projects!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>Explosive Elephant Toothpaste</p></li><li><p>Egg in Vinegar</p></li><li><p>Electrifying Lemon Battery</p></li><li><p>Making Oobleck: Liquid or Solid?</p></li><li><p>Egg Drop Project</p></li><li><p>Colors of Skittles Experiment</p></li><li><p>Cloud in a Bottle</p></li><li><p>Baking Soda Volcano</p></li><li><p>Egg in a Bottle</p></li><li><p>Plastic Milk</p></li></ul><p>Project details and more cool science projects at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/1/Popular_Science_Experiments_At_Home">Popular Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>One-Day Science Fair Project Ideas</h3><p><img src="/images2/flex/172_popular 2 (1).png" data-size="100" data-float="left" style="width:100px; float:left">Quick experiments can be a great option for a science fair project. In this list, we've highlighted one-day science fair project ideas for elementary school (easy), middle school (medium), or high school (hard). These experiments are fast and easy to do, but the science topic packs a punch!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h4>Acid-base reactions: Mixing acids with bases quickly makes carbon dioxide gas!</h4><ul><li><p><a href="https://www.all-science-fair-projects.com/project830.html"><strong>Rocket Film Canisters (easy) </strong></a>Make a rocket fly sky high with just baking soda and vinegar!</p></li><li><p><a href="https://www.all-science-fair-projects.com/project829.html"><strong>Make Your Own PH Indicator (medium) </strong></a>Learn how to make your own acid-base indicator using red cabbage and use it to test different fluids!</p></li><li><p><a href="https://www.all-science-fair-projects.com/project1327.html"><strong>Testing Antacids (hard) </strong></a>Find out which antacid can neutralize the most acid and get relief from heartburn and gastritis!</p></li></ul><h4>Chromatography reactions: Separating out colors as liquid moves through the paper!</h4><ul><li><p><a href="https://www.all-science-fair-projects.com/project826.html"><strong>Colors of Skittles Experiment (easy) </strong></a>Did you know that some Skittles have a secret rainbow hiding inside them?</p></li><li><p><a href="https://www.all-science-fair-projects.com/project678.html"><strong>Uncovering Fall Colors (medium) </strong></a>Have you ever wondered why leaves change color in the fall? Let's find out by using chromatography to separate the colors of a green leaf!</p></li></ul><h4>Heat reactions: Heat speeds things up!</h4><ul><li><p><a href="https://www.all-science-fair-projects.com/project274.html"><strong>Which Candle Burns the Fastest? (easy) </strong></a>Do you want to find out which color candle burns the fastest?</p></li><li><p><a href="https://www.all-science-fair-projects.com/project1291.html"><strong>Fire-Resistant Balloons (medium) </strong></a>Ever wondered if a balloon filled with water can withstand fire?</p></li><li><p><a href="https://www.all-science-fair-projects.com/project475.html"><strong>Can Peanuts Heat Water? (hard) </strong></a>Have you ever wondered if peanuts have enough energy to heat up water?</p></li></ul><p>Project details at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/15/Quick_Science_Fair_Projects_You_Can_Do_In_A_Day">Quick Science Fair Projects →</a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3 style="text-align: start"><strong>Testable Questions for Science Projects</strong></h3><p><img src="/images2/flex/173_popular 2.png" data-size="100" data-float="left" style="width:100px; float:left">A testable question is a question that we can answer through a science experiment. To do this, we do a control experiment, then we change one thing in the experiment to see how it affects what happens. This is how we can discover the answer to our question!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><ul><li><p>What makes a cloud form?</p></li><li><p>Can drink and food taste different just by changing its color?</p></li><li><p>Does the color of light affect photosynthesis?</p></li><li><p>Does temperature affect seed sprouting?</p></li><li><p>What makes popcorn pop?</p></li></ul><p>More <a href="https://www.all-science-fair-projects.com/science_fair_project_list/14">testable questions with their science projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h2>Science Fair Project FAQ</h2><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>What are some hands-on ways to find inspiration for my science fair project?</h3><h4>Science museums</h4><p><img src="/images2/flex/124_7.png" data-size="100" data-float="left" style="width:100px; float:left">Science museums are great places to explore if you're looking for inspiration for your next science fair project or just want to learn more about science. Science museums, natural history museums, space museums, and discovery museums are all great places to do that! You can discover all sorts of interesting things about science and find cool project ideas for science fairs.</p><p>There may be free admission days or free passes to a science museum near you! Check your credit card for offers, your local library for free museum passes, and nearby science museums for free entrance days.</p><p>So what are you waiting for? <a href="https://en.wikipedia.org/wiki/List_of_science_museums">Find a science museum near you</a> and prepare to be awed by all that you can learn there!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h4>Look around and observe</h4><p><img src="/images2/flex/117_5.png" data-size="100" data-float="left" style="width:100px; float:left">Do you know how scientists start their cool projects? They often look around and notice things that are interesting or make them curious. Then they start doing an experiment! You can do the same! Look around your school, home, or community. Is there anything that catches your attention and makes you curious? Maybe you notice something interesting or a problem that people are facing. This could be a great starting point for your science fair project!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h4>Try an Easy Science Project!</h4><p><img src="/images2/flex/144_easy square.png" data-size="100" data-float="left" style="width:100px; float:left">These science projects are easy and fun to do. You may discover a simple experiment that sparks your interest and inspires you to do more experiments, turning it into a science fair project! Check them out at <a href="https://www.all-science-fair-projects.com/science_fair_project_list/12">Easy Science Fair Projects <strong>→</strong></a></p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3 style="text-align: start"><strong>How do I start a science fair project?</strong></h3><p><img src="/images2/flex/199_science coach.png" data-size="100" data-float="left" style="width:100px; float:left">No matter if you're in high school, elementary or middle school, science fair projects are a fun and unique way to discover science by asking a question and doing a science project. We'll help you find a science fair project idea and guide you through the process of doing and presenting your science fair project with <a href="https://www.all-science-fair-projects.com/science_fair_projects_coach/science-project-coach.html">Science Fair Coach's Science Fair Project Guide</a>.</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>What should I do after I have a science fair project idea?</h3><p><img src="/images2/flex/143_intro science fair.png" data-size="100" data-float="left" style="width:100px; float:left">If you have a science fair project idea, Science Fair Coach can help you turn it into a full science fair project! Starting at the <a href="https://www.all-science-fair-projects.com/pgpage/4">Introduction to Science Fairs</a>, check out the section on <strong>How to do a science fair project</strong> for a step-by-step guide!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><p><img src="/images2/flex/203_compared to scientific method.png" data-size="100" data-float="left" style="width:100px; float:left">We'll guide you through every step of the <a href="https://www.all-science-fair-projects.com/pgpage/5">scientific method</a>, from designing and conducting your experiment to collecting and analyzing your findings.</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><p><img src="/images2/flex/202_engineering design process.png" data-size="100" data-float="left" style="width:100px; float:left">If you're working on an engineering project, you can head over to <a href="https://www.all-science-fair-projects.com/pgpage/9">The Guide to the Engineering Design Process</a>.</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><p> <img src="/images2/flex/150_science fair poster.png" data-size="100" data-float="left" style="width:100px; float:left">Finally, we'll help you showcase your results on a <a href="https://www.all-science-fair-projects.com/pgpage/7">science fair board</a> for others to learn from and enjoy.</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3 style="text-align: start"><strong>How do I make a science fair board?</strong></h3><p style="text-align: start"><img src="/images2/flex/150_science fair poster.png" data-size="100" data-float="left" style="width:100px; float:left">Your science fair board is where you show off your science fair project for everyone to see and learn from. With <a href="https://www.all-science-fair-projects.com/science_fair_projects_coach/science-project-display-board-6a.html">Science Fair Coach's step-by-step guide</a>, you can make sure your science fair board stands out and impresses at the science fair!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><hr style="clear:both; border-style:dotted" data-marker="clearFloat"><h3>Where can I find a science fair competition?</h3><p><img src="/images2/flex/179_Intro Science Fair Projects 32.png" data-size="100" data-float="left" style="width:100px; float:left">Science fair competitions are an excellent opportunity to explore science. One of the of the premier science advocacy organizations, the Society for Science, is associated with more than 400 science fair competitions in almost every US state, as well as over 70 other countries, regions, and territories. You can find out if there's a <a href="https://findafair.societyforscience.org/">Society of Science affiliated science fair</a> near you!</p><p>The <a href="http://physics.usc.edu/~gould/ScienceFairs/">The www Virtual Library: Science Fairs website</a> also has a collection of science fairs from all over the world, as well as national, state, regional, local, and virtual competitions!</p><hr style="clear:both; border-style:dotted" data-marker="clearFloat">

Microbiology is the study of tiny living things that we can't see with our eyes, like bacteria, viruses and fungi. Microbiologists study how these microorganisms grow, reproduce, and interact with their environment, and how they can affect our health.

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Antibiotic Resistance in E. coli

Hypothesis

Method & Materials

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