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Bacteriology Science Fair Project

Mouthwash and Alpha Streptococcus

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Mouthwash and Alpha Streptococcus | Science Fair Projects | STEM Projects
Does your mouthwash actually kill the bacteria living in your mouth? Alpha streptococcus is one of the most common bacteria found there. Different mouthwash brands claim to fight germs, but their ingredients vary widely. You swab your mouth to isolate alpha streptococcus on a blood agar plate. Then you grow the bacteria on Mueller Hinton plates and apply measured doses of different mouthwashes. After overnight incubation, you measure the death zone (the clear ring where bacteria could not survive) around each mouthwash sample. The larger the death zone, the more effective that mouthwash is at killing alpha streptococcus.

Hypothesis

The hypothesis is that Listerine Antiseptic Mouthwash will kill the most alpha streptococcus.

Method & Materials

You will isolate alpha streptococcus, make a suspension of the organism, inoculate plates with mouthwashes, measure zones of death with a caliper, and estimate the number of colonies within the death zones.
You will need cotton swabs, a blood agar plate, a blood agar slant, an inoculating loop, saline solution, a colorimeter, blood Mueller Hinton plates, a 10 microliter pipette, a 50 microliter pipette, an incubator, calipers, and an autoclave.

Results

The experiment showed that Listerine Antiseptic Mouthwash was the most effective in killing alpha streptococcus, with the largest death zone around the area where the mouthwash was placed.

Why do this project?

This science project is interesting because it helps to answer the question of whether or not the bacteria in our mouths is being killed by mouthwash.

Also Consider

Variations to consider include testing different types of mouthwash, testing different concentrations of mouthwash, and testing different bacteria.

Full project details

Additional information and source material for this project are available below.
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