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Renewable Energy Science Fair Project

Temperature and Methane from Cow Manure

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Temperature and Methane from Cow Manure | Science Fair Projects | STEM Projects
Does warmer storage make cow manure produce more methane gas? You fill three sealed flasks with equal amounts of manure and water. One flask goes in a refrigerator at 14 degrees Celsius. One stays at room temperature (24 degrees Celsius). The third sits on a hot plate at 34 degrees Celsius. A tube from each flask leads to an inverted water-filled measuring cylinder. As bacteria break down the manure without oxygen, methane gas collects in the cylinder. You record gas levels daily for five days. The flask kept at 34 degrees Celsius produced the most methane by a wide margin.

Hypothesis

The hypothesis is that the container stored at 34 °C will produce the most methane gas.

Science Concepts Learned

Methane

When bacteria break down organic material without oxygen, they release methane gas — and temperature controls how fast that happens. In this experiment, three sealed flasks hold equal amounts of cow manure and water at 14, 24, and 34 degrees Celsius. A tube from each flask leads to an inverted, water-filled measuring cylinder where the gas collects. After five days, the flask kept at 34 degrees Celsius produced the most methane by a wide margin.

Anaerobic Digestion

Anaerobic digestion happens when tiny living things break down waste in a sealed space with no air. In this experiment, sealed flasks hold manure and water while bacteria break down the manure without oxygen, releasing methane gas that pushes water out of an inverted measuring cylinder. The flask at 34 degrees Celsius produced the most methane, showing that warmer conditions speed up the digestion process.

Biogas

When bacteria break down organic waste in sealed, oxygen-free containers, they release methane gas — that's biogas forming in real time. In this experiment, cow manure and water sit in sealed flasks, each connected by a tube to an inverted, water-filled measuring cylinder. As bacteria digest the manure without oxygen, methane collects in the cylinder. Temperature drives how fast that happens: the flask at 34 degrees Celsius produced the most methane by a wide margin, while the refrigerator flask at 14 degrees barely registered. Warmer conditions speed up bacterial activity, which is why the hot-plate flask outpaced both the refrigerator and room-temperature setups.

Renewable Energy

Methane gas from cow manure is a renewable energy source because bacteria keep producing it as long as organic material is available. When three sealed flasks of manure and water sit at 14, 24, and 34 degrees Celsius, a tube from each leads to an inverted water-filled measuring cylinder where the gas collects. After five days, the flask at 34 degrees Celsius produced the most methane by a wide margin.

Method & Materials

You will need three Erlenmeyer flasks, three corks, a nail, three tubes, cow manure, tap water, three measuring cylinders, three basins, a refrigerator, a hot plate, and a ruler.
You will observe the amount of methane gas released into each measuring cylinder every day for 5 days.

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Results

The results show that the manure in the Erlenmeyer flask that was stored at 34 °C produced the most methane gas. This proves that the hypothesis was correct.

Why do this project?

This science project is interesting because it explores the effects of temperature on the decomposition of dairy waste and the production of methane gas.

Also Consider

Consider repeating the experiment using rotting vegetables or other organic material.

Full project details

Additional information and source material for this project are available below.

Related video

These videos explain the science behind this project and demonstrate key concepts used in the experiment.
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