Does adding phosphate to pond water lower the oxygen level? Phosphate feeds algae growth. Too much algae can use up dissolved oxygen (the oxygen mixed into water that fish need to breathe).
You fill eighteen jars with tap water and add algae to each one. Three jars get no phosphate and serve as the control. The rest get increasing amounts of a phosphate solution. You place all the jars under a grow light and measure dissolved oxygen each day.
The control jars held the most oxygen. Jars with more phosphate drops generally had less dissolved oxygen. Even small amounts of added phosphate made a measurable difference.
Hypothesis
The hypothesis is that the phosphate will have an effect on the level of oxygen in the pond water.
Algae grow in water and make food from light — and phosphate feeds that growth. When too much algae accumulates, it can deplete the dissolved oxygen that fish need to breathe. This experiment fills eighteen jars with tap water and algae, then adds increasing amounts of a phosphate solution to most of them. Three jars get no phosphate and serve as the control. All jars go under a grow light, and dissolved oxygen levels get measured each day. The control jars hold the most oxygen, while jars with more phosphate generally show less — even small amounts make a measurable difference.
Phosphate feeds algae growth, and when algae thrive, they consume the dissolved oxygen that fish need to breathe. This experiment tests that relationship directly: eighteen jars of tap water each receive one milliliter of algae, then varying amounts of a phosphate solution. Control jars get none. All jars sit under a grow light while you measure dissolved oxygen daily with a dissolved oxygen meter. Jars with more phosphate generally ended up with less dissolved oxygen — even small amounts made a measurable difference.
Method & Materials
You will fill eighteen jars with tap water and one milliliter of algae, label them, and place them under a growth light. You will measure the amount of dissolved oxygen daily using a dissolved oxygen meter.
You will need eighteen jars, tap water, algae, a growth light, and a dissolved oxygen meter.
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The results showed that the jars with the constant had more oxygen than the jars with the detergents. The jars with ten drops of the detergent solution had the most oxygen, followed by the jars with twenty, thirty, forty, and fifty drops.
Why do this project?
This science project is interesting because it shows how adding a small amount of phosphate can have a big effect on the oxygen levels in water.
Also Consider
Variations to consider include testing different types of phosphate, testing different amounts of phosphate, and testing different types of water.
Full project details
Additional information and source material for this project are available below.