What happens when yeast runs out of oxygen but still has sugar to eat? It switches to fermentation. That process produces alcohol and carbon dioxide instead of using oxygen.
You place dried baker's yeast in a sealed plastic bag. Inside a second perforated bag, you crush 150 grams of grapes to release the juice. The juice mixes with the yeast. A pH strip taped inside tracks acidity changes. Over the next hour, gas fills the bag and foam appears.
You test the gas with a glowing splint. Carbon dioxide snuffs it out. The pH drops as the juice turns more acidic. Leave the bag open for a few days and bacteria convert the alcohol to vinegar.
When yeast runs out of oxygen but still has sugar to eat, it switches to fermentation — producing alcohol and carbon dioxide instead. You seal dried baker's yeast and crushed grapes together in a plastic bag, so the yeast uses grape sugar as a substrate, yielding carbon dioxide and ethanol. A pH strip taped inside tracks acidity changes as the juice turns more acidic. Gas fills the bag and foam appears.
You place 5 grams of dried baker's yeast in a sealed plastic bag, and inside a second perforated bag you crush 150 grams of grapes to release the juice. As the juice mixes with the yeast, gas fills the bag and foam appears over the next hour. Testing the gas with a glowing splint confirms it: carbon dioxide snuffs it out. The pH drops as the juice turns more acidic.
When yeast runs out of oxygen but still has sugar to eat, it switches to fermentation — a process that produces carbon dioxide and ethanol instead of using oxygen. You place dried baker's yeast in a sealed plastic bag alongside the juice from 150 grams of crushed grapes, with a pH strip taped inside to track acidity changes. Over the next hour, gas fills the bag and foam appears.
Yeast cells rely on glycolysis — the first step cells use to break sugar apart and release energy — to feed on grape juice. When dried baker's yeast meets the juice from 150 grams of crushed grapes in a sealed bag, it splits the grape sugar into smaller molecules that fermentation then converts into alcohol and carbon dioxide. Over the next hour, gas fills the bag and foam appears, visible signs that the yeast broke the sugar apart and released energy from it.
You will need two self-locking plastic baggies, one which has perforations, a heavy-duty freezer bag, 5 grams of dried bakers yeast, a pH indicator strip, and 150 g of grapes.
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The experiment showed that yeast can use grape sugar as a substrate for cellular respiration, resulting in the production of carbon dioxide and ethanol. The most interesting observation was that the carbon dioxide gas produced extinguished the splint.
Why do this project?
This science project is interesting and unique because it demonstrates the process of fermentation, which is used to make many different types of alcoholic beverages.
Also Consider
Experiment variations to consider include testing different temperatures to determine the best temperature for yeast enzyme activity, and allowing air to reach the juice after a few days to observe the conversion of alcohol to acetic acid due to bacterial action.
Full project details
Additional information and source material for this project are available below.