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Food Science Science Fair Project

The Digestion of Protein

The Digestion of Protein | Science Fair Projects | STEM Projects
Ever wonder how the acid in our stomachs affects the digestion of proteins? Let's find out! We'll be testing different pH levels to see how they affect the digestion rate of a protein called albumin.


The hypothesis is that a pH level of 4 will digest the protein fastest.

Method & Materials

You will place a protein, albumin, into four test tubes for each of the seven pH levels being used. Then, you will add the buffer solution for each pH level into their respective tubes. After a period of ten minutes, you will add Ninhydrin Isopranol to one of the tubes for each pH level. This step will be repeated every ten minutes. Then after being centrifuged, the solutions will be observed for color change and the absorbance will be recorded using a spectrophotometer at 580 nm.
You will need albumin, buffer solution, Ninhydrin Isopranol, test tubes, a centrifuge, and a spectrophotometer.


The data showed that as the pH level increased, the rate of digestion also increased rapidly. From a pH level of eight and up, the digestion was almost immediate. At lower pH levels, the rate of digestion was rather slow, which did not support the original hypothesis.

Why do this project?

This science project is interesting because it shows how the pH level of acid in our stomachs affects the digestion of proteins, which is an important part of our digestive process.

Also Consider

Experiment variations to consider include testing different proteins and testing different pH levels.

Full project details

You can find additional information and details for this science fair project here. Have fun exploring!

Related video

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