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Bacteriology Science Fair Project

Bioluminescent Bacteria as Pollution Sensors

Hard
Bioluminescent Bacteria as Pollution Sensors | Science Fair Projects | STEM Projects
Can glowing bacteria tell you how polluted water is? Some ocean bacteria produce their own light through a process called bioluminescence. When pollutants harm the bacteria, their glow fades. You transfer bioluminescent bacteria into test tubes using a sterilized metal loop. Then you add different pollutants one at a time: - hair gel - 3-in-1 oil - tap water - Hudson River water To measure each effect, you count how many sheets of paper it takes to block the glow. Hair gel cut the light by 75 percent. Tap water and river water each reduced it by 20 percent. The 3-in-1 oil had no effect at all.

Hypothesis

The hypothesis is that pollutants will decrease the amount of light produced by the bacteria.

Method & Materials

You will transfer the bacteria to test tubes, then add pollutants to see if the light they produce decreases.
You will need bioluminescent bacteria, metal transferring loop, Hudson River water, tap water, hair gel, 3 in 1 oil, Bunsen burner, sparker, rubbing alcohol, napkin, pen/pencil, paper, test tubes, goggles, and a dropper.

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Results

Our hypothesis was proven correct and at the same time proven wrong. We found that the pollutants decreased the amount of light the bacteria gave off, except for the 3 in 1 oil.

Why do this project?

This science project is interesting because it shows how pollutants can affect the environment, even on a microscopic level.

Also Consider

Experiment variations to consider include testing different pollutants, or testing the bacteria in different environments.

Full project details

Additional information and source material for this project are available below.

Related video

These videos explain the science behind this project and demonstrate key concepts used in the experiment.
In this science fair project video, a student tested bioluminescent bacteria and observed that its fluorescence decreased when flame retardants were added.
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