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Nicotine and Cancer
Hard
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This science fair project will explore the effects of nicotine on human colorectal adenocarcinoma cells. We will use a phosphatase assay kit to measure the rate of cell proliferation.
Hypothesis
The hypothesis is that the human colorectal adenocarcinoma cells will grow at a faster rate after being exposed to nicotine.
Method & Materials
You will extract tissue samples from a tissue culture flask and place them in 12 well plates. You will then prepare nicotine solutions in three beakers and add them to the well plates. Finally, you will incubate the plates for three days and measure the rate of cell proliferation using a phosphatase assay kit.
You will need human colon colorectal adenocarcinoma tissue, a tissue culture flask, a phosphatase assay kit, well plates, nicotine, distilled water, a spatula, a digital weighing scale, three beakers, a measuring cylinder, and a pipette.
Results
The results showed that the presence of nicotine encouraged cell proliferation, proving our hypothesis to be true. Despite the warnings about the dangers of smoking, most smokers still find it difficult to quit.
Why do this project?
This science project is interesting because it explores the effects of nicotine on cancer cells, which is an important topic in today's society.
Also Consider
This science project can also be repeated using other tissue, such as human prostrate carcinoma samples. Another variation is to expose the tissue to cigarette smoke.
Full project details
You can find additional information and details for this science fair project here. Have fun exploring!Related videos
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